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KMID : 0613819990090020169
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Journal of Life Science
1999 Volume.9 No. 2 p.169 ~ p.175
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Anaerobic Acid Tolerance Responase in Salmonella typhimurium
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Abstract
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Salmonella typhimurium can encounter a wide variety of environments during its life cycle. In nature, S. typhimurium can experience and survive dramatic acid atresses that occur in diverse ecological niches ranging from pond water to phagolysosomes. These survival emchanism is aquried by the Acid Tolerance Response(ART) in Salmonella. The ART of S. typhimurium is a complex inducible ohenomenon in which exposures to slight or moderate low pH will produce a stress response capable of protecting the organism against more esvere acid challenges. ATR in Slamonella has two different systems that dre called RpoS dependent and independent. We found that ATR in anaerobic was showed RpoS independent because rpoS$\Omega$Ap had ATR as S. typhimurium UK1. Using the P22 MudJ(10mM, pH4.5), we isolated LF487 aatA::MudJ which showed acid sensitive in anaerobic condition. aatA locus was determined at 12 min on Salmonella Genetic Map. The survival rate of aatA mutant was showed significantly diminished at pH4.3 than virulent wild type Salmonella in ancerobic condition(5% CO$_{2}$, 5% H$_{2}$, 90% N$_{2}$). Therefore isolated gene was confirmed important gene for anaerobic ATR system.
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